Composite

Part:BBa_K539563:Design

Designed by: Shu-Han Chang   Group: iGEM11_NCTU_Formosa   (2011-09-27)

vioC+a RBS+lacI repressor+RNA thermometer+tetR+double terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1716
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2481
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Built in vioC(Part:BBa_K539513), strong expressing RBS, LacI, 37’celcius regulator RBS, tetR and Terminator(Part:BBa_K539558).

Source

The vioC sequence is cloned from the Cambridge 2009 iGEM team .And the part which repress Ptet when the temperature up to 37'Celius is designed by 2010 iGEM NCTU_Formosa.

References